comparison of real-time polymerase chain reaction and conventional cell culture for detection of influenza a in tabriz, iran
نویسندگان
چکیده
background influenza type a (h1n1) causes an epidemic disease, resulting in thousands of deaths throughout the world. objectives our aim was comparing the efficacy of two different methods in isolating the influenza (h1n1) virus including cell culture and real-time pcr in tabriz and suburbs. patients and methods of throat swab samples, 220 were collected in viral transport medium (vtm) from patients with a suspected influenza virus infection referred to hospitals and clinics of tabriz university of medical sciences. all the samples were examined through virus culturing in madin-darby canine kidney epithelial cells (mdck line) and also real-time polymerase chain reaction (rt-pcr). the collected data were analyzed by spss version 16. results rt-pcr detected 41 cases of influenza a (h1n1), compared with the virus isolation methods that detected 18 cases. conclusions rt-pcr provided a sensitive and specific route compared with virus isolation. in addition, urgent planning for the vaccination program of influenza a is suggested, which can definitely prevent the spread of virus in this part of iran.
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عنوان ژورنال:
avicenna journal of clinical microbiology and infectionجلد ۱، شماره ۲، صفحات ۰-۰
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